EPIGENETIC PROFILING

 

Epigenetics is an emerging frontier of science that involves the study of changes in the regulation of gene activity and expression that are not dependent on gene sequence. HuGe-F provides services to measure epigenetic features, such as DNA-methylation.

HuGe-F supports epigenetic studies by providing help in designing your study and guide preparation of samples and quality control, to study DNA methylation on a genome-wide or smaller scale. We encourage researchers to contact us at an early stage to discuss the details of their project

 

 

We offer the following assays for methylation analysis:

 

 

1. Genome-wide techniques: Illumina Infinium MethylationEPIC BeadChip array (MethylationEPIC array)

2. Targeted techniques: Amplicon Bisulphite Sequencing and Pyrosequencing

 

 

The MethylationEPIC array by Illumina is a cost-effective solution for epigenome-wide association studies (EWAS). The array gives single-base methylation information for over 850.000 CpGs throughout the human genome. It is suitable for a variety of human tissues, allows for low DNA input and offers a combination of comprehensive, expert-selected coverage, high sample throughput, and affordable price.

 

The Illumina MethylationEPIC beadchip is an update of the popular Humanmethylation450 platform beadchip. The EPIC array contains >90% of the original Methylation450 Beadchip content plus an additional 350.000 CpGs in enhancer regions. The content of the Beadchip was selected by a global consortium of epigenomic experts.

 

The EPIC array has a comprehensive genome-wide coverage of the methylome, featuring:

  - -   >95% of CpG islands

  - -   sites outside of CpG islands

  - -   Non-CpG methylated sites identified in human stem cells

  - -   Differentially methylated sites identified in tumor versus normal

  - -   FANTOM5 enhancers

  - -   ENCODE open chromatin and enhancers

  - -   DNase hypersensitive sites

  - -   miRNA promoter regions

 

 If you are interested and want to know more about the possibilities and details, please send an email to dr. Gaby van Dijk.

 

 

We offer data-analysis services for analyzing epigenetic data.

 

Please contact us for further information.